NISA’UL KAMILAH, 061511133009 (2019) EFFECT OF ADDITION OF GREEN TEA (Camellia sinensis) EXTRACT IN EGG YOLK SKIM MILK EXTENDER ON POST-THAW SPERMATOZOA QUALITY OF BOER CROSSBRED GOAT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research was conducted to investigate the effect of addition of green tea (Camellia sinensis) extract in extender on post-thaw spermatozoa motility, viability, plasma membrane integrity, and chromatin integrity. Sample was collected from ejaculate of Boer crossbred goat. The semen sample were diluted in egg yolk skim milk extender supplemented with green tea extract of 0 (T0) as control, 0.05 (T1), 0.1 (T2), and 0.15 (T3) mg/100 ml extender, respectively. Data analyzed using Analisis of Variant (ANOVA) One Way followed with Duncan to determine significant differences between treatments. The result showed that T2 had the highest post-thaw spermatozoa motility, viability, plasma membrane integrity, and lowest DNA fragmentation compared to other treatments. The result of the study suggest that semen samples diluted in egg yolk skim milk extender with green tea extract dose of 0.1 mg/100 ml extender had the highest semen quality

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