SITI NUR ROHMAH, 061511133134 (2019) PENGARUH PEMBERIAN MADU LEBAH (Apis dorsata F.) TERHADAP KADAR KALSIUM TULANG MANDIBULA TIKUS PUTIH (Rattus norvegicus) YANG DIOVARIOHISTEREKTOMI. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this study was to evaluate the effects of giving honey bee (Apis dorsata F.) on calcium levels of mandibular bone in ovariohysterectomized rat as animal models of osteoporosis. The samples were 20 female rats wich were divided into 5 treatment groups with 4 replications. SHAM was sham operated rats as negative control, OH was ovariohysterectomized rats without treatment as positive control, wheares AD1, AD2, AD3 was ovariohysterectomized rats with treatment Apis dorsata 1 g/KgBW, 2 g/KgBW, 4 g/KgBW for 84 days. After 84 days of treatment, the rats left mandibular bone were harvested, weighed and kept in 10% Neutral Buffered Formalin (10% NBF) until analysis. Calcium levels were calculated using proximate analysis. The data was analyzed using ANOVA and Duncan as the Post-Hoc Test. The results showed a decreased of bone calcium levels on ovariohysterectomized rats, the highest results were in the SHAM group and the lowest results were in the AD3 group. Based on this results, it can be concluded that honey bee (Apis dorsata) can not maintain calcium levels of mandibular bone in ovariohysterectomized white rats (Rattus norvegicus

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