Widya Saraswati and Ira Widjiastuti and Mandojo Rukmo and Dian Agustin Wahjuningrum (2017) The expression of HMGB1 in Dentin Pulp Complex Induced by Resin Monomer HEMA. In: Proceeding International Medical Device and Technology Conference 2017. Universiti Technologi Malaysia, Johor, Malaysia. ISBN 978-967-0194-93-6

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Abstract

High mobility group box protein 1 (HMGB1) is a nonhistone nuclear protein that can stimulate innate immunity and drive the pathogenesis of various inflammatory diseases and can be triggered by infections, tissue damage and metabolic imbalance. HMGB1 can exit cells during stimulation of the inflammasome during a process called pyroptosis that induced by non microbial particles such as asbestos and chemical agents such as resin monomer 2-hydroxyethtyl dimethacrylate (HEMA). This condition will drive innate immune response towards invading pathogens and cellular damage in dentin pulp complex. However, it is still unclear how resin monomer HEMA could trigger the expression of HMGB1 to activate innate immun response in the pulp. The objective of this research is to identify the molecular mechanism which activate HMGB1 in dentin pulp complex induced by resin monomer HEMA. We used nine healthy whistar rat teeth on in vivo experiments. Tooth cavity was applied with HEMA (Sigma Aldrich) in concentration of 0,016μg/ml, then covered with glass ionomer cements (Fuji IX LC, GC Japan) as cement. Teeth were extracted after 24, 48 and 72 hours. The teeth were decalsificated using EDTA for 8 weeks and the paraffin block were made after the teeth were cut 5 milimicron by microtome. Immunohistochemistry staining was applied and using antibody anti HMGB1 to investigate regulation these antibody on odontoblast pulp cells in dentin pulp complex. The sample were analyzed statistically by Anova and Tukey HSD. HEMA upregulated the expressions of HMGB1 in odontoblast cells of dentin pulp complex after 12,24 and 48 hours. HMGB1 induced innate immunity response in dentin pulp complex

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