Rimayanti, - (2013) The Role Of External Heat Shock Protein 70 (HSP70) Supplementation on Expression Of Caspase3 In Oocyte During Vitrification. In: Proceeding International Seminar The Role of Veterinary Science to Support Millenium Development Goals and The 12th Asian Association of Veterinary Schools Congress. Faculty of Veterinary Medicine Universitas Airlangga, Surabaya, pp. 429-433. ISBN 978-602-70438-0-0

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Abstract

The cryopreservation of animal and human oocytes has potential prospect for the development of Assisted Reproductive Technology (ART). One of the techniques used for oocyte cryopreservation is vitrification procedure to avoid the critical temperature and reduce cell damage caused by the formation of ice crystals on oocyte cryopreservation. The aim of this study was to evaluate the role of external HSP70 supplementation on the expression of pro-apoptosis related gen Caspase 3 in oocyte during vitrification. In Vitro Matured bovine oocytes were exposed to vitrification cryoprotectant consisting of 40% Ethylene Glycol (EG) + 0.3M Sucrose (S) + different dose of HSP70 supplementation (0.25 ~g/ml, 0.5 ~g/ml, 1.0 ~g/ml, and 2.5~g/ml). Oocytes were kept in Open Pulled Straw, and then plunged into liquid nitrogen rapidly. After keeping the oocytes for 2-4 weeks, the frozen oocytes were thawed in a 30111 C water bath and washed twice in O.5M Sucrose. Examination of caspase 3 expression was done to fresh oocytes, in vitro maturated oocytes, and post-thawing oocytes with different dose of HSP70 supplementation by using immunofluorescencetechnique. In conclusion, supplementation of HSP70 into vitrification medium supressed the activation of caspase 3. Keywords: bovine oocyte, vitrification, HSP70, caspase 3

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