Widjiati and Rimayanti and Arief Budiono and Agus Setiadi (2010) Peran Transforming Growth Factor ẞ terhadap Tingkat Kematangan dan Kejadian Apoptosis Oosit Sapi pada kultur In Vitro. Jurnal Veteriner, 11 (2). pp. 92-98. ISSN 1411-8327/e-ISSN : 2477-5665

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Abstract

Low productivity of in vitro embryo production at blastosis level appears to be as a result of imperfect oocyte maturation which causes imperfect oocyte growth, and in turn will affect the embryo growth. In addition to hormonal factor, growth factor plays significant role in maturation process of oocytes. As growth factor might has a significant role during maturation process, a study was conducted to determine whether transforming growth factor (TGF β) isolated from oocytes of cumulus complex is required for co culture and in vitro embryo production. Oocytes were collected from follicles with the diameter of 3-5 mm and > 5 mm. Then the oocytes were cultured for 22 hours at 38.5o with 5% CO2 atmosphere in tissue culture medium (TCM) 199 supplemented with 5 µg/mg luteonizing hormone (LH), 3% bovine serum albumin (BSA) 50 µg/ml gentamycin sulfat and three different levels of TGF β (12,85 pg/ml, 25,7 pg/ml and 38,55 pg/ml). The oocyte maturation and number of apoptosis cells were examined. The result showed that oocyte maturation in medium supplemented TGF β at the dose of 38,55 pg/ml was better than in that at dose of 12,85pg/ml or 25,7 pg/ml. The best maturation was observed at metaphase II stage of oocyte development. No apoptosis was observed during maturation of oocytes. Supplementation of TGF β at the dose of 38,55 pg/ml in culture medium increased the oocyte maturation without causing a significant apoptosis in vitro. Keywords: TGF β, apoptosis, cumulus complex

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