VALENTIKA FITRIA SETYANINGTYAS, 051511133009 (2019) AKTIVITAS ASETILKOLINESTERASE INHIBITOR DARI SUBFRAKSI ETIL ASETAT SPONS Agelas nakamurai IN VITRO. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this study was to examine the acetylcholinesterase inhibitory activity of the ethyl acetate subfractions of Agelas nakamurai collected from Tabuhan Island, Banyuwangi, East Java. The fractionation was performed on vacuum liquid chromatography by using gradient elution with mobile phase combination of dichloromethane and methanol in order of increasing polarity; to produce 11 subfractions. The AChE inhibitory activity assay was carried out according to the Ellman's method. The test results show that subfractions 2 to 6 have % inhibition ≥ 50%. The IC50 values of subfractions 2 to 6 were determined, which showed that subfraction 3 gave the strongest activity with IC50 value of 3,78 ± 1,10 μg/mL. Phytochemical screening was conducted on subfractions 2 to 6 by using thin layer chromatography method, visualized with Dragendorff and Anisaldehyde H2SO4 dyes. The results indicated the presence of alkaloid and terpenoid in the active subfractions. These results suggest that these compounds may contribute to the inhibitory activity of ethyl acetate subfractions Agelas nakamurai.

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