Reza Mahendra Yudha Permana and Widjiati and Budi Utomo and Tjuk Imam Restiadi (2015) Pengaruh Suplementasi Insulin Transferrin Selenium (ITS) pada Media Maturasi TCM-199 terhadap Diameter Oosit Sapi. Veterinaria Medika, 8 (3). pp. 245-250. ISSN 1979-1305

	Text (Jurnal) Bukti C 20 Pengaruh Suplementasi Insulin Transferrin .....pdf Download (1MB)
	Text (Peer Review) Peer Review Bukti C20.pdf Download (1MB)

Abstract

Insulin Transferrin Selenium (ITS) has been used in vitro maturation system to support in vitro maturation of oocyte. The study aims was to determine role of Insulin Transferrin Selenium to bovine oocyte were matured in vitro against improved oocyte diameter. Maturation process used TCM-199 medium which added by FSH 100 IU/ml, LH 100 IU/ml, BSA 355 and ITS (Insulin Transferrin Selenium). ITS dose which used in culture medium are different for each treated group. The treated groups are Pp (0 ug/ml), P1 (10 ug/ml), Ps (15 ug/ml), and P3 (20 ug/ml). Oocytes were incubated in CO) incubator with CO, 5Y9, Humidity 95Y6 and 38.5"C due 22 hours. The result showed that ITS supplementation in maturation medium has no significantly difference (p20,05) for ali treated groups (Po. Pi, P5, and P3) of oocyte diameter improvement. Conclusion of ITS supplementation in maturation medium TCM-199 can't increase oocyte diameter. Keywords: oocyte, in vitro maturation, Insulin Transferrin Selenium, diameter

Actions (login required)

View Item