SHOFFA ILLIYYIN ERFANI, 051511133242 (2019) AKTIVITAS ANTIOKSIDAN EKSTRAK n-HEKSANA, ETIL ASETAT, DAN METANOL Caulerpa racemosa DARI PULAU KANGEAN DAN PULAU MANDANGIN JAWA TIMUR. Skripsi thesis, Universitas Airlangga.

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Abstract

In the study the antioxidant activities of n-hexane, ethyl acetate, and methanol extracts of Caulerpa racemosa were tested by using DPPH method performed on spectrophotometer. The absorbance was measured at wavelength 517 nm. Vitamin C was used as positive control. The results showed that n-hexane extract of C.racemosa from Mandangin Island gave a strong antioxidant activity at IC50 of 94,94 ± 24,33 μg/mL, while the other 5 extract only showed weak to moderate activities at IC50 of 137-1441 μg/mL. The phytochemical screening was also conducted for all extracts by using TLC methods. The mobile phase used was n-hexane : ethyl acetate (1:1) and was visualised with anisaldehyde-H2SO4 for terpenoid, FeCl3 for polyphenol, ammonia for flavonoid and Dragendorff for alkaloid. The results indicated that all C.racemosa extracts contain terpenoid and polyphenol. In addition there are flavonoid in the n-hexane extract from Kangean Island and Mandangin Island, while alkaloid are found in all three extracts from Mandangin Island.

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