EVI NUR’AIN MURANDANI, 051511133170 (2019) VALIDASI METODE KROMATOGRAFI GAS DETEKTOR SPEKTROMETRI MASSA UNTUK PENETAPAN KADAR VITAMIN D3 DALAM SEDIAAN TABLET MULTIVITAMIN. Skripsi thesis, Universitas Airlangga.

	Text FF.KF.17-19 Mur v abstrak.pdf Download (62kB)
	Text FF.KF.17-19 Mur v daftar isi.pdf Download (27kB)
	Text FF.KF.17-19 Mur v daftar pustaka.pdf Download (68kB)
	Text (FULLTEXT) FF.KF.17-19 Mur v.pdf Restricted to Registered users only until 14 November 2022. Download (1MB) | Request a copy

Abstract

Vitamin D3 is a fat-soluble vitamin that is available with other vitamins in multivitamin tablet. As a health supplement, the amount of vitamin D3 in multivitamin tablet is 400 IU or equivalent to 10μg in each tablet. Due to the low level of vitamin D3 contained in this complex sample matrix, a specific and sensitive method is needed to ensure the product quality, safety and efficacy. Therefore, Gas Chromatography – Mass Spectrometry is used in this study, because of its high sensitivity, high resolution, andspecifically detection ofthe analytesat low concentration in samples. The objective of the present study was to validate the GC-MS methods in terms of specificity, linearity, range, accuracy and precision. The proposed method was carried out without derivatization. The optimum condition was reached by oven temperature program with initial temperature of 150°C for 2 minutes, then increased by 25°C/minute to the final temperature of 280°C for 10 minutes. The time needed for running is about 17.20 minutes. From the result of the method validation, the retention time of vitamin D3 was 8 minutes with resolution of 2.86. The method showed a linear response (r=0.997) with Vxo of 3.54%. The average of percent recovery of the sample was 98.71% with relative standard deviation of 2.57%. Detection and quantitation limits were found to be 0.61 ppm and 1.85 ppm, respectively.

Actions (login required)

View Item