AZISZIA INSANYA LAMAKLUANG, 051511133071 (2019) PENGARUH KONSENTRASI GLUTARALDEHID TERHADAP POROSITAS SCAFFOLD NA-DIKLOFENAK BERBASIS GELATIN-KITOSAN-PEG 400 SEBAGAI PENGGANTI KARTILAGO (dibuat dengan metode pre-freezing). Skripsi thesis, Universitas Airlangga.

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Abstract

Scaffold making is composed of natural polymers such as chitosan and gelatin which are widely developed in the field of cartilag tissue engineering. Chitosan-gelatin-PEG 400 polymer scaffold has biocompatible, biodegradable, non-toxic, non-immunogenic properties with cartilage matrix and can be formed as a local Sodium diclofenac delivery system. Scaffold preparations as a local sodium diclofenac delivery system and cartilage replacement are formed having a porous texture that will facilitate the process of diffusion, proliferation, differentiation of chondrocyte cells, nutrient supply processes, and the process of releasing sodium diclofenac in the joint cavity. In the scaffold crosslink agent glutaraldehyde was used with a concentration of 0.00%; 0.25%; 0.50%; 0.75%; 1,00%; 2.50% with the addition of 0.5 mL / 16 mL of chitosan-gelatin-PEG 400 mixture. Making scaffold using the prefreezing method with a drying temperature of -56 ± 5 ° C for 24 hours, then observing the pore diameter using SEM Model TM 3000 Tabletop Microscope 5-15 kV and porosity percentage using the liquid media transfer method (Buffer phosphate buffer pH 7.45) into the empty scaffold cavity. The observational data were statistically analyzed using the ANOVA ONE WAY test with a degree of confidence (α) = 0.05. Based on the results of statistical tests, it was concluded that increasing concentrations of added glutaraldehyde would increase the porosity percentage of chitoid-gelatin-gelatin-PEG 400-based scaffold implants significantly. The optimal concentration of glutaraldehyde to form the percentage of porosity in accordance with the requirements (> 80%) was in sample C (GA 0.50%) of 106.94 ± 9.38%, while the addition of glutaraldehyde concentration did not have a significant effect on pore diameter. Pore diameter in accordance with the requirements (100-400 μm) was formed at a concentration of 0.50% GA of 135.48 ± 89.70 μm.

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