DIAS NATASIA MASTIKA PUTRI, 051511133196 (2019) VALIDASI METODE KLT-DENSITOMETRI UNTUK PENETAPAN KADAR GLUKOSAMIN HCl, L-ARGININ HCl DAN GLISIN SECARA SIMULTAN DALAM SEDIAAN SUPLEMEN KESEHATAN. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Validation of thin layer chromatography-densitometry for simultaneous determination of glucosamine HCl, L-arginine HCl and glycine in dietary supplement has been done. The aim of the present study was to obtain a valid thin layer chromatography-densitometry for simultaneous determination of glucosamine HCl, L-arginine HCl and glycine in dietary supplement. The selective separation of glucosamine HCl, L-arginine HCl and glycine was obtained using a mixture of 1-buthanol: glacial acetic acid: water (3:1:1) as mobile phase with ninhydrin as visualization reagent. The resolution (Rs) between glucosamine HCl and glycine was 1.3 and Rs between glycine and L-Arginine HCl was 4.6. A good linearity was achieved with the correlation coefficient (r) for each compounds were higher than 0.99 with the relative process standard deviation value (Vxo) was not more than 5%. Recoveries of glucosamine HCl, L-arginine HCl and glycine were 99.97% ± 1.60; 100.30% ± 1.51 and 99.90% ± 1.39, respectively. The relative standard deviation (RSD) value was less than 2% for each compounds. The method showed good results and fulfill the validation requirements.

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