Citrawati Dyah Kencono Wungu and Mochamad Amin and S. Eriaty N. Ruslan and Priyo Budi Purwono and Ulfa Kholili and Gwenny Ichsan Prabowo and Ummi Maimunah and Poernomo Boedi Setiawan and Maria Inge Lusida and Soetjipto and Retno Handajani (2019) Hepatitis B Virus X Gene Mutation With Predominance A1762T+G1764A Double Mutation on Chronic Liver Disease Patients in Surabaya, Indonesia. Malaysian Journal of Biochemistry & Molecular Biology, 01. pp. 27-31. ISSN 15112616

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Abstract

Background: HBV infection is a major problem worldwide, especially in developing countries including Indonesia. Mutations in the HBV gene X are commonly found in patients with CLD, especially in cirrhosis and hepatocellular carcinoma. Mutations in the X gene can cause loss of stability, increased transactivation function, and decreased anti-apoptotic ability of HBx protein. Aim: The aim of this study was to detect HBV X gene mutations in CLD patients in Surabaya. Methods: This was a cross sectional research taking samples at Dr. Soetomo General Hospital, Surabaya, Indonesia. This study used nested PCR by targeting HBV X gene. Samples showing positive HBV DNA PCR results were followed by sequencing and X gene mutation analysis by comparing sequencing results with reference strains. Results: In this study, 30 samples of CLD patients with positive HBsAg in Dr. Soetomo Surabaya were obtained. From the results of the multiple alignments, 12/30 samples (40%) had mutations on HBV X region which overlapped with Core Promoter region. There were 3 types of substitution mutations on HBV X gene (C1632T, T1753A/C/G, A1762T, and G1764A) with the dominant mutation types were A1762T and G1764A mutations, in which both mutations were found together as double mutation. Conclusion: X gene mutations were found in 40% CLD patients in Surabaya with the dominant mutation was in the form of double mutation A1762T and G1764A in 30% CLD patients in this study. The mutation was found mostly in advance stage of CLD.

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