PRESTALIA DWI RACHMAWATI, 061514253007 (2019) ANALISIS FILOGENETIK GEN PARSIAL PENYANDI PROTEIN PERMUKAAN VIRUS AVIAN INFLUENZA SUBTIPE H9N2 YANG DIISOLASI DARI AYAM PETELUR DI BEBERAPA WILAYAH JAWA TIMUR PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

Avian influenza virus subtype H9N2 has become established in domestic poultry in Asia and Africa. Ninety-nine cases were observed in various provinces in Indonesia. Clinical sign, pathologic lesions and egg production were recorded. The aim of this study is to find out the homology of nucleotide sequence of surface Protein H9N2 influenza virus isolate from Chickens Layer in east Java. Confirmation was made using virus isolation, reverse transcriptase PCR (RT-PCR), and squencing. To construct hemaglutinin (HA) phylogeny, the secondary data of Eurasian lineages were downloaded from GenBank. For Neuraminidase (NA), squences with the higest similarities with data sequences from GenBank found in this study. Phylogenic was inferred using neighbor- joning method in Mega 7.0 package. Homology analysis of nucleotide sequences and amino acid sequences was performed on the BLAST program at NCBI. The result showed that the nucleotide sequence of the HA-encoding gene identified in this study shared 96.96% squence identify with that of A/duck/Hongkong/Y280/1997, while it’s neurominidase-encoding gene sequences shared 94.37% identities with that A/duck/Hongkong/Y280/1997. The phylogeny swows that the HA of AIV-H9N2 found in this study forms distinct cluster with some vietnam and China’s sequence data. We conclude that AIV-H9N2 widespread in districts in east java.

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