Fihiruddin and Wayan Tunas Artama and Tri Wibawa and Ni Made Mertaniasih (2019) Expression of Imunoglobulin, Granzyme-B and Perforin Againts Ag85A and AG85B Proteins of Mycobacterium Tuberculosis in Balb/C Mice. African Journal Infectious Diseases, 13 (02). pp. 13-20. ISSN 2006-0165

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Abstract

Background: Ag85 is a protein that may maintain survival of M.tuberculosis in intracellular parts of host cells and is considered as a virulence factor. The expression of Ag85 protein can stimulate proliferation and differentiation of B cells and T cells in patients with tuberculosis. This research aimed to determine the ability of Ag85A and Ag85B proteins in activating the response of antibodies, granzyme-B and perforin in Balb/c mice.Methods: 25 male Balb/c mice were assigned into five groups. Group I was treated with adjuvant, group II with BCG vaccine (Biofarma), group III with a combination of BCG and Ag85A, group IV with a combination of BCG and Ag85B and group V with a combination of BCG, Ag85A and Ag85B. Concentrations of Immunoglobulin G, granzym-B and perforin were examined using ELISA and the number of CD8+ T-cells and NK T-cells were checked by flowcytometry.Results: The highest concentration of imunoglobulin G was found in group-V with 62.49±5.4327 ng/ml. The highest mean number of CD8+ T-cells, NK T-cells, granzyme-B and perforin was found in group–IV with 4.32%, 1.03%, 35.11±1.7789 pg/ml and 6.19±0.2235 pg/ml respectively. The results of One-Way Anova test showed that there were significant differences in immunoglobulin responses, with p <0.05. The expressions of granzym-B and perforin were higher in mice treated with combination of BCG and recombinant proteins.Conclusions: Ag85 protein can be combined with the BCG vaccine to improve protection against M. tuberculosis infection.

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