Tutiek Purwanti and Widji Soeratri and Muhamad Zainudin (2018) Characterization of Nisin Microspheres with Combination Matrix Sodium alginate – Gelatin. International Journal of Pharma Research and Health Sciences, 6 (6). pp. 2838-2843. ISSN 2348-6465

	Text (PEER REVIEW) C-10 Reviewer.pdf Download (1MB)
	Text (SIMILARITY) C-10 Result.pdf Download (1MB)
	Text (FULLTEXT) C-10 Artikel.pdf Download (840kB)

Abstract

The aim of this research is to characterize nisin microsphere were made by ionotropic gelation method aerosolization technique with sodium alginate-gelatine combination as matrix with total polymer concentration 2,5% and calcium chloride 1.5 M as the crosslinker solution. The composition of this nisin microsphere formulas are F1 (10:0), F2 (9:1), F3 (8:2), F4 (7:3), F5(6:4), and F6 (5:5). The interaction between polymer and nisin was confirmed by the changes in the intensity and wave number on the FT-IR spectra. X-Ray Diffraction of nisin microsphere showed amorf characteristic. Characterization of nisin microsphere include organoleptic,yield value, moisture content, morfology, particle size, and swelling index. The organoleptic of all nisin microspheres are white fine powder,tasteless and odorless.Nisin microspheres are spherical particles with smooth surface as displayed by Scanning Electron Microscope.Yield value of nisin microsphere were F1(67,85%), F2(46,97%), F3(57,84%), F4(41,34%), F5(49,95%), F6(31,61%), Moisture content of nisin microsphere areF1(12,37%), F2(10,72%), F3(11,67%), F4(10,26%), F5(11,90%), and F6(9,24%).Nisin microspheres have mean particle diameterF1(5,66µm), F2 (4,05µm), F3 (3,75µm), F4(3,60µm), F5(3,53µm), F6(3,35µm).The presence of gelatin as a matrix resulted in changes of swelling patterns from the microspheres, which were sloping increasingly and occurred at longer time intervals when compared to those without gelatin. This phenomenon increased by the time ofthe increasing gelatin concentration and the decreasing of the concentration of sodium alginate in the matrix of nisin microspheres

Actions (login required)

View Item