Nirawati Pribadi and Ruslan Effendy and Diva Amelia Ruslianda (2017) The Difference Between the Amount of Glucose as the Product of Metabolism of Glucosyltransferase Enzyme Streptococcus Mutans In Neutral pH and Optimal pH. In: Proceeding International Medical Device and Technology Conference (IMEDITEC) 2017. Universiti Technology Malaysia., Johor, pp. 121-123. ISBN 978-967-0194-93-6

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Abstract

Background:Caries happens due to the production of organic acid from the sugar metabolism, it cause a decrease in oral pH. The changes of pH affects the formation of glucan as the product of metabolism GTF enzyme S. mutans. Purpose: To investigate whether neutral pH (7) will decrease the amount of glucose compared to pH (5.5, 6.0, and 6,5). Methods: The S. mutans’s was cultured in BHI and centrifuged to obtain its supernatants. Six samples for each four group, consisted of 0.9 ml of 0.25 M sucrose in 0.2 M buffer, 0.1 ml of GTF enzyme, and buffer solution (pH 5.5, 6.0, 6.5, and 7) with total volume of 2ml (incubated), were then measured by using HPLC . Result: The amount of glucose (pH 7) are significantly less than samples (pH 5,5 and 6,5). Conclusion: The amount of glucose (pH 7) is less in acid pH, due to the changes of enzyme’s charge and denaturation.

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