Muhammad Miftahussurur and A. F. Syam, A. F. Syam and Dadang Makmun, Dadang Makmun and Iswan Abbas Nusi, Iswan Abbas Nusi and Lukman Hakim Zein, Lukman Hakim Zein and Zulkhairi, Zulkhairi and Fardah Akil, Fardah Akil and Willi Brodus Uswan, Willi Brodus Uswan and David Simanjuntak, David Simanjuntak and Tomohisa Uchida, Tomohisa Uchida and Pangestu Adi, Pangestu Adi and Amanda Pitarini Utari, Amanda Pitarini Utari and Yudith Annisa Ayu Rezkitha, Yudith Annisa Ayu Rezkitha and Phawinee Subsomwong, Phawinee Subsomwong and Nasronudin, Nasronudin and Yoshio Yamaoka, Yoshio Yamaoka (2015) Helicobacter pylori virulence genes in the five largest islands of Indonesia. Gut Pathogens, 7 (1). ISSN 17574749
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Miftahussurur-Helicobacter pylori virulence genes in the five largest islands of Indonesia.pdf Download (1MB) |
Abstract
Background: It remains unclear whether the low incidence of gastric cancer in Indonesia is due to low infection rates only or is also related to low Helicobacter pylori pathogenicity. We collected H. pylori strains from the five largest islands in Indonesia and evaluated genetic virulence factors. Methods: The genotypes of H. pylori virulence factors were determined by polymerase chain reaction (PCR)-based sequencing. Histological severity of the gastric mucosa was classified into 4 grades, according to the updated Sydney system. Results: A total of 44 strains were analyzed. Forty-three (97.7 ) were cagA-positive: 26 (60.5 ) were East-Asian-typecagA, 9 (20.9 ) were Western-type-cagA, and 8 (18.6 ) were novel ABB-type, most of which were obtained from Papuan. EPIYT sequences were more prevalent than EPIYA sequences (P = 0.01) in the EPIYA-B motif of all types of cagA. The majority of cagA-positive strains (48.8 , 21/43) had a 6-bp deletion in the first pre-EPIYA region. Subjects infected with East-Asian-type-cagA strains with a 6-bp deletion had significantly lower inflammation and atrophy scores in the corpus than those infected with Western-type-cagA strains (both P = 0.02). In total, 70.4 of strains possessed the vacA s1m1 genotype and 29.5 were m2. All strains from peptic ulcer patients were of the iceA1 genotype, which occurred at a significantly higher proportion in peptic ulcer patients than that in gastritis patients (55.3 , P = 0.04). The double positive genotype of jhp0562/â-(1,3)galT was predominant (28/44, 63.6 ), and subjects infected with this type had significantly higher inflammation scores in the corpus than those with the jhp0562 negative/â-(1,3)galT positive genotype (mean median; 1.43 1 vs. 0.83 1, P = 0.04). There were significant differences in cagA and pre-EPIYA cagA type, oipA status, and jhp0562/â-(1,3)galT type among different ethnic groups (P < 0.05). Conclusions: In addition to a low H. pylori infection rate, the low incidence of gastric cancer in Indonesia might be attributed to less virulent genotypes in predominant strains, which are characterized by the East-Asian-type-cagA with a 6-bp deletion and EPIYT motif, a high proportion of m2, dupA negative or short type dupA, and the jhp0562/â-(1,3) galT double positive genotype. © 2015 Miftahussurur et al.
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| Uncontrolled Keywords: | Helicobacter pylori, Indonesia, Virulence factors | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research |
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| Divisions: | Artikel Ilmiah > SCOPUS INDEXED JOURNAL | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Depositing User: | PPJPI | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Date Deposited: | 02 Mar 2022 03:03 | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Last Modified: | 02 Mar 2022 03:03 | |||||||||||||||||||||||||||||||||||||||||||||||||||
| URI: | http://repository.unair.ac.id/id/eprint/94617 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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