Ni Made Mertaniasih and Nurul Wiqoyah and Kuntaman and Manik Retno Wahyunitisari and Deby Kusumaningrum (2013) Polymerase Chain Reaction of Mycobacterium tuberculosis GYRB Gene Region for Rapid Screening Tes of Pumonary Tuberculosis. Folia Medica Indonesiana, 49 (1). pp. 16-20. ISSN 0303-7932

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Abstract

gyrB gene of Mycobacterium tuberculosis have conserved and specific DNA region with high copy number. Primer base in conserved and specific DNA region increases the accuracy of nucleic acid amplification method. Determining of tuberculosis diagnosis accurately and rapidly would be useful to appropriately determine the therapy. The objective of this study was to determine sensitivity and specificity of the PCR of gyrB gene region to detect Mycobacterium tuberculosis compared with gold standard method using Lowenstein Jensen (LJ) medium. Thirty sputum samples from pulmonary Tuberculosis suspects were examined using standard culture method in LJ medium and PCR gyrB gene region. Using PCR we positively found gyrB gene region of Mycobacterium tuberculosis in 21 sputum samples, similar to that if we used the gold standard method, with 100% sensitivity and 100% specificity. In conclusion, PCR of Mycobacterium tuberculosis gyrB gene region has a high accuracy, a rapid screening method for TB laboratory diagnosis

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