Muhammad Yunus and Suwarno and Lucia Tri Suwanti and Mufasirin (2009) Characterization and the Increase of Chicken Interferon-gamma Production as a Measure of T-cell Responses to Eimeria tenella Antigens. Media Kedokteran Hewan, 25 (2). pp. 82-89. ISSN 0215-8930

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Abstract

Characterization and the increase of chicken interferon-gamma (ChIFN-y)production were investigated in Eimeria tenella (E. tenella) infected chicken. Twenty poultry broilers at three weeks old were divided into two groups. The first group was control and those chickens were not infected anything. The second group was infected with 4 x 10 of E. tenella. Spleen cells ofboth the groups were cultured and induced mitogen. This study was to characterize ChIFN-y protein through extraction technique of supernatant protein of the culture medium of chicken spleen cell that was induced Con-A by SDS-PAGE. The result of characterization showed that molecule weight of ChIFN-y protein was 17 kDa. Then, Optical Density (OD) of ChIFN-y protein was detected ELISA reader 450 nm of both E. tenella uninfected and infected chickens was 1.29 and 1.95, respectively, whereas, concentration of ChIFN-y protein of both those groups was 10.312 pg/ml and 15.569 pg/ml, respectively. Key words: E. tenella, chicken interferon-gamma

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