Yuliana Pratiwi and Heni Puspitasari and Lucia Tri Suwanti and Mufasirin (2016) Production and Characterization of Immunoglobuline Yolk as Anti Antigen Membrane Toxoplasma gondii. Indonesian Journal of Tropical and Infectious Disease, 6 (2). pp. 29-33. ISSN 2085-1103, e-ISSN 2356-0991

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Abstract

Toxoplasma gondii is an obligate parasite intracellular which can infection human and other mammalian. Immunoglobulin Y technology offers several advantages better than antibody production in mammals. This research is aimed to get immunoglobulin Y from egg yolk, and to find the characterization of immunoglobuline Y according to molecular weight by SDS PAGE and targeted protein with antibodies using Western Blot. This research divided from many step: culture tachyzoites of T. gondii fromintraperitoneal fluid, preparation of membrane antigen tachyzoite of T. gondii, then immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blot. The result of this research showed that immunoglobulin Y from egg yolk can produced antibody against protein membrane of T. gondiiandprofile protein immunoglobuline Y according SDS PAGE has molecular weight 179,8 kDa. Immunoglobuline Y was analyze by Western Blot can recognize antigen epitope of T.gondii on molecular weight 35,7kDa and 78,8 kDa. Keywords: Toxoplasma gondii, anti membrane T.gondii, immunoglobulin Y anti membrane

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