DEWI VENDA E., 050710077 (2011) VALIDASI METODE KCKT UNTUK PENENTUAN GENISTEIN DALAM PRODUK SUSU KEDELAI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Over 80 va rieties of mostly agriculturally important legumes were surveyed as sources of the genistein metabolites. Genistein is a phytoestrogen (estrogen - like chemical compound present in plants) that binds to estrogen receptors and has both weak estrogenic and weak anti - estrogenic effects. Genistein has been found in soy milk. In vitro studies with high concentrations of genis tein demonstrate inhibition of cell proliferation, while in vivo studies demonstrate that genistein inhibits chemically - induced mammary cancer rats. Genistein was determined using LiChrospher RP 18 column chromatography with a gradient elution of acetonitr ile and 10 % acetic acid. The whole process was monitored using high performance liquid chromatograph (HPLC), equipped with photodiode array detector. The HPLC chromatogram with UV at 260 nm showed that the purity of genistein in soy milk sample was greate r than 99% to the standard . In the determination of linearity , were obtained a good linear relationship. The recoveries were in the range 90,67 – 111,76%. Concentration of genistein in soymilk were 5,14 x 10 - 4 % . The validated method can be utilized as a suitable method for the quality control of genistein in soy milk product.

Item Type:	Thesis (Skripsi)
Additional Information:	KKB KK FF 257 11 Ven v
Uncontrolled Keywords:	ALCOHOLS
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	05. Fakultas Farmasi > Farmakognosi Fitokimia
Creators:	Creators NIM DEWI VENDA E., 050710077 UNSPECIFIED
Contributors:	Contribution Name NIDN / NIDK Thesis advisor Herra Studiawan, Drs. , Apt., MS UNSPECIFIED Thesis advisor Riesta P, Dr. . , SSi.,M.Si.,Apt. UNSPECIFIED
Depositing User:	Dwi Marina
Date Deposited:	26 Jan 2012 12:00
Last Modified:	14 Jul 2016 07:59
URI:	http://repository.unair.ac.id/id/eprint/9545
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