Ernawati and Reny I'tishom and Sri Agus Sudjarwo (2020) Role of MDA, SOD and GPx Expression on Protective Mechanism of Xanthone Against 2-Methoxyethanol-Decreased Number of Sertoli Cell in Mice. Interciencia Journal, 45 (1). pp. 104-114. ISSN 03781844

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Abstract

Background: 2-Methoxyethanol (2-ME) can increase Reactive Oxygen Species (ROS) production that cause damages to the male reproductive organs such as Sertoli cell. Xanthone is one of the natural antioxidant that can neutralize ROS. Objective: The purpose of this research was to determine the protective mechanism of xanthone on 2-ME-induced Sertoli cell oxidative damage. Methods: The research used 35 male mice divided into 5 groups: control group (mice were given daily with water purified by distillation); 2-ME group (mice were given daily with 2-ME 200 mg/kg BW orally once in a day for 35 days); and the treatment group ( mice were given the xanthone 60 mg, 120 mg, and 240 mg/kg BW orally once in a day for 38 days, and on the 3th day, were given 2-ME 200 mg/kg BW one hour after the xanthone administration). After 38 days, the testis tissues were collected to evaluate the immunohistochemical of the expression of malondialdehyde (MDA), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) in Sertoli cell. Testis tissue was also taken to histological evaluations of Sertoli cell number. Results: The immunohistochemical evaluation showed that 2-ME administration significantly decreased the expression of SOD, GPx, and increased the expression of MDA in Sertoli cell. The histopathological evaluations showed that 2-ME also significantly decreased Sertoli cell number. However, administration of xanthone significantly increased the expression of SOD, GPx, and decreased the expression of MDA on Sertoli cell in immunohistochemical evaluation. Xanthone significantly increased Sertoli cell number in histopathological evaluation. Conclusion: In conclusion, our results indicate that xanthone as antioxidant agent is able to increase Sertoli cell number in mice treated with 2-ME through decreased MDA expression, and increased both SOD and GPx expression

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