Herluinus Mafranenda Dwi Nugrahananto and Indah Listiana Kriswandini and Ester Arijani R. (2014) Antimicrobial proteins of Snail mucus (Achatina fulica) against Streptococcus mutans and Aggregatibacter actinomycetemcomitans. Dental Journal (MajalahKedokteran Gigi), 47 (1). pp. 31-36. ISSN 1978-3728

	Text (ARTIKEL) 07.Antimicrobial proteins of Snail mucus.pdf Download (401kB)
	Text (SIMILARITY) 13.Antimicrobial proteins of Snail mucus (Achantia fulica) against Streptococcus mutans and Aggregatibacter actinomycetemcomitans.pdf Download (1MB)
	Text (PEER REVIEW) Dr.Indah (Antimicrobial).pdf Download (915kB)

Abstract

Background: Achasin and mytimacin-AF are proteins of snail mucus (Achatina fulica) which have antimicrobial activity. Snail mucus is suspected to have other proteins which have antimicrobial activity against Streptococcus mutans and Aggregatibacter actinomycetemcomitans the oral pathologic bacteria. Purpose: The study were aimed to characterize the proteins of snail mucus (Achatina fulica) that have antimicrobial activities to Streptococcus mutans and Actinobacillus actinomycetemcomitans, and to compared the antimicrobial effect of achasin and mytimacin-AF. Methods: The sample of study was the mucus of snails which were taken from Yogyakarta Province. The isolation and characterization of protein were conducted by using SDS-PAGE method, electro-elution, and dialysis. Nano drop test was conducted to determine protein concentration. The sensitivity test was conducted by using dilution test, and followed by spectrophotometry and paper disc diffusion tests. Results: The study showed that proteins successfully characterized from snail mucus (Achatina fulica) were proteins with molecular weights of 83.67 kDa (achasin), 50.81 kDa, 15 kDa, 11.45 kDa (full amino acid sequence of mytimacin-AF) and 9.7 kDa (mytimacin-AF). Based on the dilution test, Achasin had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans. But the paper disc diffusion test result showed that Achasin had antimicrobial activities against Streptococcus mutans and Aggregatibacter actinomycetemcomitans, while mytimacin-AF had no antimicrobial activities. Conclusion: The proteins with molecular weights of 50.81 kDa, 15 kDa, 11.45 kDa were considered as new antimicrobial proteins isolated from snail mucus. Achasin, had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans.

Actions (login required)

View Item