Paparan zat besi pada ekspresi protein spesifik extracellular polymeric substance biofilm Aggregatibacter actinomycetemcomitans

Marchella Hendrayanti W and Indah Listiana Kriswandini (2014) Paparan zat besi pada ekspresi protein spesifik extracellular polymeric substance biofilm Aggregatibacter actinomycetemcomitans. Dental Journal (MajalahKedokteran Gigi), 47 (2). pp. 103-109. ISSN 1978-3728

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09.PaparanZatBesi pada Ekspresi Protein.pdf
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14.paparan zat besi pada ekspresi protein spesifik extracellular polymeric substance biofilm Aggregatibacter actinomycetemcomitans.pdf
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Abstract

Background: The study of biofilms bacteria could be an alternative of preventive treatment in reducing prevalence of aggressive periodontitis in the community, because biofilm protects the bacteria from environmental conditions, including the attack of immune system and antimicrobial. Aggregatibacter actinomycetemcomitans is a major cause of bacterial aggressive periodontitis. Purpose: This study aims to examine the iron exposure to specific protein expression of extracellular polymeric substance (EPS) of Aggregatibacter actinomycetemcomitans biofilm. Methods: Protein containing EPS biofilm was isolated from cultures of A.actinomycetemcomitans. The protein was processed through several procedures: electrophoresis , electroelution , immunization of rabbits , serum isolation , and purification of antibodies. After the Western blotting procedure the antibody was used. Protein containing EPS biofilms exposed to iron, then once again isolated from cultures of A. actinomycetemcomitans. The electrophoresis and Western blotting were done on the isolated protein. Results: The result showed that the the expression of specific proteins in EPS biofilm decreased in response to iron exposure. Conclusions: Iron exposure could influenced the specific protein expression in EPS biofilm of Aggregatibacter actinomycetemcomitans.

Item Type: Article
Uncontrolled Keywords: Iron, specific protein, extracellular polymeric substance, biofilm, Aggregatibacter actinomycetemcomitans , specific protein, extracellular polymeric substance, biofilm, Aggregatibacter actinomycetemcomitans
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM
Marchella Hendrayanti WUNSPECIFIED
Indah Listiana KriswandiniNIDN0030106303
Depositing User: Rudy Febiyanto
Date Deposited: 26 Aug 2020 03:34
Last Modified: 29 Dec 2020 02:52
URI: http://repository.unair.ac.id/id/eprint/96101
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