NI KADEK LINTIA LAKSMI, 050911207 (2013) PURIFIKASI DAN KARAKTERISASI METABOLIT DARI FRAKSI NO.150-200 DAN FRAKSI NO.10-13 ETIL ASETAT JAMUR ENDOFIT Cladosporium oxysporum DARI Aglaia odorata Lour. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The profiles of the Cladosporium oxysporum metabolites from fraction no. 150-200 and no.10-13 were analized by TLC-Densitometry method. Fraction no.150-200 were fractionated by column chromatography with gradient eluen system and yielded 360 vials. Fractions obtained were collected according to their TLC patterns to give 18 major fractions (A-R). Fraction F that had 5.2 mg of crystal contained 1 intensive spot. Then fraction F was purificated by recrystallization and performed analytical TLC to ascertain purity by 4 different solvent systems. The result was fraction F contained 3 spots with Rf value 0.58; 0.67 and 0.75. The spot with Rf value 0.75 was intensive spot, the ultraviolet spectra showed maximum absorption at 279 nm. These fraction had positive result after sprayed by using anisaldehyde- H2SO4, cerri- H2SO4, vanillin- H2SO4, iodine vapour. Fraction no.10-13 were fractionated by column chromatography with isocratic eluen system and yielded 278 vials. Fractions obtained were collected according to their TLC patterns to give 9 major fractions (A-I). By TLC with CHCl3:EtOAc=1:4 as eluen system, fraction C that had 2.2 mg of crystal contained 1 intensive spot with Rf value 0.77. This spot showed maximum absorption at 277 nm. These fraction had positive result after sprayed by using anisaldehyde- H2SO4, cerri- H2SO4, vanillin- H2SO4 and iodine vapour.

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