Muhammad Rif’at Akhsan (2020) Histopatologis Hepar Ayam Petelur Yang Diinfeksi Avian Pathogenic Escherichia Coli (Apec) Setelah Diberi Kombinasi Ekstrak Sambiloto (Andrographis Paniculata Nees.) Dan Meniran (Phyllanthus Niruni Linn.). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The purpose of the research is to know combination of meniran extract (Phyllanthus niruri Linn.) and sambiloto extract (Andrographis paniculata Nees.) to the histopatological liver layer of chicken infected with APEC (Avian Pathogenic Escherichia coli). This research used twenty four of thirty two weeks old layer hens, divided into six treatment groups, each of group contain of four layer hens. Treatment groups consists of P(-) (suspension of CMC Na 0.5% + aquadest), P(+) (Suspension of CMC Na 0.5% + E. coli bacteria with concentration 108 CFU/ml/kgBW), P(Exract) (combination of meniran extract (Phyllanthus niruri Linn.) with 20% concentration and sambiloto extract (Andrographis paniculata Nees.) with 20% concentration + aquadest), P1, P2, and P3 (each of group given with meniran extract (Phyllanthus niruri Linn.) and sambiloto extract (Andrographis paniculata Nees.) 30% and 10%, 20% and 20%, also 10% and 30% + E. coli bacteria with 108 CFU/ml/kgBW concentration). Observation with hepatocyte cell necrosis, degeneration of hepatocyte cell and inflamation portals. Data analysis was performed with the kruskall-wallis test and continued with the mann-whitney test. This research showed significant difference (p<0.05) between each treatment group. The conclusion of this research showed that combination meniran extract (Phyllanthus niruri Linn.) and sambiloto extract (Andrographis paniculata Nees.) can effect the liver histopatology with the best combination to reduce the level demage due to APEC infection is 20% : 20%.

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