Cendranata Wibawa Ongkowijoyo (2020) Perbandingan Degradasi Biofilm Enterococcus Faecalis Akibat Paparan Laser Dioda Dengan Fotosensitiser Chlorophyll Dan Psoralens. Other thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Enterococcus faecalis are dominant in root canal infection and can survive harsh conditions such as high pH, scarce nutrients, and heat. They are able to form mature biofilm readily and able to resist disinfection approaches. Photo-activated disinfection with photosensitizer and laser irradiation is a viable alternative to eliminate E. faecalis biofilm. Objective: This study aimed to investigate and compare E. Faecalis biofilm degradation using photo-activated disinfection with chlorophyll or psoralens photosensitizer with 405 nm diode laser irradiation. Methods: This study used E. Faecalis biofilm grown in laboratory. Chlorophyll or psoralens were applied as photosensitizer, and then 405nm diode laser was irradiated as long as 30 or 60 seconds. The effectiveness of biofilm degradation was measured with optical density using microplate reader. Results: Chlorophyll with 30 seconds of laser irradiation results in 23.6% biofilm degradation, whereas psoralens with 60 seconds of laser irradiation results in 41.3% biofilm degradation. One-way ANOVA test results in significant difference of biofilm degradation effectiveness amongst all groups. Conclusion: Psoralens with 60 seconds of 405nm diode laser irradiation yielded the most Enterococcus faecalis biofilm degradation compared to other groups.

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