MOH. EKO INDRIYANTO, 050810191 (2013) PENGARUH PEMBERIAN VANADIUM PENTAOKSIDA TERHADAP EKSPRESI PHOSPHOENOLPYRUVATE CARBOXYKINASE PADA JARINGAN HEPAR MENCIT DENGAN DIABETES MELLITUS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The present study was designed to investigate the influence of vanadium pentoxide toward lowering blood glucose level and supressing gluconeogenesis in hepatic tissues in alloxan-induced diabetic mice. Twenty five mices were divided into five groups i.e. normal, diabetic, and three dosages of treatment vanadium pentoxide groups (0,5; 1,0; and 5,0 mg/KgBW). Diabetic mices were induced by alloxan monohydrate intra peritoneally at the dose of 150 mg/KgBW. Diabetic state occurred on the 3rd day after alloxan administration and it was showed increasing blood level until 433 ± 126 mg/dL. Orally administration of vanadium pentoxide for 7 days after diabetic condition were significantly reduce of blood glucose level (P<0.05). At the day 10th after treatment, mices were sacrified and the hepatic tissues were harvested for Haematoxyllin eosin staining and immunohistochemistry study using phosphoenolpyruvate carboxykinase (PEPCK) antibodies. Morphologically vanadium pentoxide can make dilatation of sinusoid. The result showed that vanadium pentoxide on groups with dosages 0,5 and 1,0 mg/KgBW were significantly suppressed expression PEPCK enzyme compared positive group (p<0,05). On group with dosage 5,0 mg/KgBW showed that expression of PEPCK enzyme not significantly different compared with positive group.

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