Arumentin Diana and Tutiek Purwanti and Isnaeni (2019) Utilization of Lactobacillus Acidophilus FNCC-0051 Microencapsulation: Potential Benefit of Giving Combination of Sodium Alginate and Gelatin to Attributes and Role of Probiotic Against Staphylococcus Aureus. Indian Journal of Public Health Research and Development, 10 (9). pp. 1410-1415. ISSN 0976-0245

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Abstract

Background: The use of Lactobacillus acidophilus as an antimicrobial is still not optimal, even though this type of probiotic has benefits for skin health. One of bacteria causesthe turnaround cause effects of the skin is Staphylococcus aureus. Objective: To evaluate the effect combination of sodium alginate and gelatin matrix 2.25%: 0.75% to the characteristics, viability, and antibacterial activity of probiotic microparticles. Method: The experimental study used the ratio of sodium alginate and gelatin 2.25%: 0.75% (FI), 3% sodium alginate (FII) and 3% gelatin (FIII). Tests were carried out to assess viability (TPC method), making microparticles by extrusion method and antibacterial activity (agar diffusion method). The data obtained was analyzed by statistical tests. Results: Particle size distribution was obtained, including 8.85 μm (FI), 9.69 μm (FII) and 5.40 μm (FIII). The viability of probiotics after being made microparticles is still in the range of probiotic minimum requirements despite a decrease. The lowest decrease in viability was 1.32% ± 0.06 (FI) and the highest decrease in viability was 14.77% ± 1.21 (FIII) which was significantly different (p <0.05). The lowest antibacterial activity test 9.27 ± 0.19 mm (FIII) and the highest antibacterial activity 10.83 ± 0.51 mm (FI) which was significantly different from FIII. Conclusion: Thecombination of sodium alginate and gelatin 2.25%: 0.75% can increase the role of Lactobacillus acidophilus probiotic as antimicrobial against Staphylococcus aureus and can be an alternative in preventing infection.

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