Lilik Maslachah, - and Eddy Soewandojo, - and Mufasirin, - and Yuni Priyandani, - and Loeki Enggar Fitri, - and Didi Candradikusuma, - (2010) Molecular Characterization of pfcrt Gene for Detection Chloroquine Antimalarial Drug Resistance at Clinic Isolates Plasmodium Falciparum. Proceeding International Seminar “Strategies For The Control Prevention of Zoonotic Diseases". pp. 59-62. ISSN 978-602-8967-00-6

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Abstract

Research of molecular characterization pfcrt gene to detection chloroquine antimalarial drugs resistance at clinic isolates Plasmodium falci parum, aims to prove and lay open mechanism and cause antimalarial drugs resistance at local clinic isolat of Plasmodium falcifarum through determination of pfmdrl gene. M ethod of the research: Making thin and thick blood films from malaria patient for microscopic inspection to determine density and identify parasite . In vitro culture to get Plasmodium falci parum parasite Isolate. Drug susceptibility testing to determine minimum inhibiting concentration (MIC) to antimalarial drugs. DNA extraction . Amplification pfcrt, gene with PCR technique. Result of research sensitivity test by in vitro Chloroquine 500 ng!ml fo r the Plasmodium fa lcipa rum isolates from malaria patient used micro test method modifyfrom WHO by using flat bottom well 96 (WHO, 1990) Result of IC50 test got Chloroquine drug can only be pursued with big dose 500 nglml. Result of PCR by using specific primary for the amplification gene region coding Pfcrt gen at 479- 630 bp genes Plasmodiumfalcifarum can be expressed so that result of this can be used as marker for the detection anti malarial drugs resistance. The concluded: M inimum concentration to pu rsue growth of parasite equal to 50%. Chloroquine require big cosentrasition to pursue growth parasite 500ng!ml. Pfcrt gene Plasmodium falcifarum can be used as marker to detect chloroquine antimalarial drugs resitance. Suggestion: Result of gene from PCR have to be continued with squencing to know mutation gene. Furthermore gene marker to make diagnostic kit as rapid test so that can give input and benefit to government evaluating and updating antimalarial drugs treatment p olicies, and give input to doctor in therapy with correct antimalarial drugs to malarial pati ents. Key words: chloroquine, resistance, pfcrt gene

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