Antibody Titers in The Sheep which were Immunated Antigen of Whole Protein from Third Instrar Larvae Musca domestica

B Ariantini and Hermin Ratnani and Epy Muhammad Luqman and Poedji Hastutiek (2019) Antibody Titers in The Sheep which were Immunated Antigen of Whole Protein from Third Instrar Larvae Musca domestica. IOP Conference Series Earth and Environ. Sci. 217. pp. 1-7.

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Official URL: https://iopscience.iop.org/article/10.1088/1755-13...

Abstract

This research aimed to know antibody titers in the sheep which were injected antigen of whole protein from third instar larvae Musca domestica by Enzyme-Linked Immunosorbent Assay (ELISA), that giving the antigen could cause increasing antibody titers in the sheep. A male sheep about two years old were immunated with whole protein from larvae Musca domestica which was added Freund’s Complete Adjuvant and done three times injection again (booster) with range of time once-two weeks and addition of Freund’s Incomplete Adjuvant. Taking sheep blood before and after immunitation was done to get serum as composition of ELISA test. Data which were got in this research, tabulated with descriptive analysis. The result of this research showed that there was a little of increasing antibody titers. The most titers at booster 1, then gradually had drastic decreasing. Based on the result of ELISA test can be concluded that antigen of whole protein from larvae Musca domestica can’t stimulate immune response in the sheep. Keyword : Antibody, Sheep, Immunated Antigen, Protein, Musca domestica

Item Type: Article
Uncontrolled Keywords: Antibody, Sheep, Immunated Antigen, Protein, Musca domestica
Subjects: S Agriculture > SF Animal culture > SF371-379 Sheep. Wool
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Parasitologi Veteriner
Creators:
CreatorsNIM
B AriantiniUNSPECIFIED
Hermin RatnaniNIDN0006045802
Epy Muhammad LuqmanNIDN0013126703
Poedji HastutiekNIDN0003116103
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 16 Sep 2020 02:31
Last Modified: 16 Sep 2020 02:31
URI: http://repository.unair.ac.id/id/eprint/98769
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