Indah Listiana Kriswandini (2017) Periodental Diseases Detection With Aggregatibacter actinomy cetemcomitans Biofilm. In: The 7th Temu Ilmiah Nasional and 4th Joint Scientific Meeting in Dentistry, 5-7 Oktober 2017, Surabaya.

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	Text (ABSTRAK) ABSTRAK DETEKSI PERIODONTAL DISEASES DENGAN BIOFILM Timnas-dikonversi.pdf Download (187kB)
	Text (PEER REVIEW) periodental desease.pdf Download (8MB)
	Text (ARTIKEL2) INDAH LISTIANA KRISWANDINI.pdf Download (9MB)

Abstract

Background : Infectious bacteria always produce biofilm which one of its functions is for attachment. Aggregatibacter actinomycetemcomitans (A.a) bacteria are the causes of periodontal diseases that produce biofilms with specific molecular weight characteristics of biofilms. Similarly, the character of A.a biofilm exposed to antibiotics. Purpose :The objective of this study was to characterize the molecular weight of Biofilm bacteria A.a that exposed to antibiotics Metronidazole, Clindamycin and Amoxillin.Methods :The first step of this study was conducted by growing the Aa bacteria isolated from Periodontal infection plaque. A.a is grown on Lauria Berthani broth media induced with each one type of antibiotic. Induction of antibiotics used was Metronidazole (150 mg); Clindamycin (75 mg) and Amoxillin (125 mg). The growing biofilm is a biofilm produced by A.a to the antibiotic induction. This biofilm performed running SDS PAGE determines Molecular Weight and then saliva patient that exposed antibiotic as primer antibodi performed western blotting Result :A.a exposure with three different antibiotics produces different biofilm proteins: 37.6 kDa molecular weight (induced biofilm A.a with Metronidazole); 48.1 kDa molecular weight (induced biofilm A.a with Clindamycin) and 60.2 kDa molecular weight (induced biofilm A.a with Amoxillin)Conclusion : Protein band with60.2 kDa molecular weight can be used to detect the presence of A.a in patient saliva

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