Simon Martin Manyanza Nzilibili and Moh. Kurniadi Hendry Ekodiyanto and Pudji Hardjanto and Ahmad Yudianto (2018) Concentration and Purity DNA Spectrophotometer: Sodium Monofluorophosphate forensic impended effect. Egyptian Journal of Forensic Sciences, 8 (34). pp. 1-7. ISSN 20905939

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Abstract

Background: A number of factors have been identified to affect DNA analysis for forensic purposes. SMFP compound which is constituted in toothpaste is one of those factors identified to cause this effect. The impact of this compound in forensic science is far evidenced to contaminate, inhibit and destroy biological samples. Toothbrush, one of preferable forensic evidences to recover biological sample in contact (brushing); might not be useful in identifying an individual if the person used toothpaste of SMFP compound. The SMFP reaction leads to inhibited band visualisation, concentration and purity contamination that lead to a failed analysis. This study presents experimental observational findings on the detrimental effects of the compound on DNA concentration and purity profiled from sample recovered from toothbrush. Results: Using spectrophotometer with complementary findings from electrophoresis, it was found that among the ten samples analysed one sample had extreme DNA concentration of 371 μg/ml with minimal purity measurement A260/A280 ratio of 1.25. Concentration analysed portrays the detrimental effect that damage and destroy DNA molecules into increased segmented molecules. Purity readings suggest the lowered amount of intact DNA molecules that would be enough to make into PCR. Also, PCR and electrophoresis method portrayed inhibition and failed visualisation of sex gene band contrast. Twofold phenomena emerged: limited DNA targeted locus for electrophoresis and DNA ionic irresponsive interactions. These are suggested by a twofold causation; one being non-polymerisation of the targeted DNA region and the other was destructed molecular structure acted by SMFP respectively. Conclusion: SMFP compound impedes concentration and purity of DNA physical samples, thereby affecting the physical sample by hindering forensic profiling through damaging DNA molecule to a no applicability and acceptability state. These findings verdict contributes to the understanding of SMFP constituted toothpaste action on biological sample during DNA forensic analysis.

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