Ahmad Yudianto and Simon Martin Manyanza Nzilibili (2019) Effect of Room Temperature and Length of Exposure to recover quality DNA from Earphone Swab-Derived 126bp and 143bp Mitochondrial DNA at D-loop region. Journal of Punjab Academy of forensic Medicine & Toxicology, 19 (1). pp. 100-107. ISSN 0972-5687
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Effect of Room Temeperature and Length of Exposure to recover quality DNA from Earphone Swab-Derived 126bp and 143 Mitochondrial DNA at D-loop region.pdf Download (522kB) |
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Effect of room Temperature.pdf Download (2MB) |
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Effect of Room Temeperature and Length of Exposure to recover quality DNA from Earphone Swab-Derived 126bp and 143 Mitochondrial DNA at D-loop region.pdf Download (2MB) |
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Abstract
Introduction: Exploration of crime scene devices left over and its surrounding is of significance in forensic identification. The significance is due to its association and/or potential harbor of biological evidence; ie., blood, blood spots, semen patches, vaginal swabs, buccal swabs, bones, or trace evidence. Such harboring of evidence from humankind proposes traceability under Locard’s principle of contact. Materials and Methods : Processing of cerumen expelled or traces logged from the 30 used earphones were subjected to 0, 1, 7, 14 and 20 days room temperature exposure. Results : Length of exposure has a significant effect on decreasing the level of DNA steadily. Visualization of the results of PCR on 143bp (HVS I, nt 16268-16410) mtDNA D-loop region showed a positive (+) detection only for day 1 (4 [66.67%] samples) and day 7 (3[50%] samples). Visualization of the results of PCR on the 126bp (HVS II, nt 34-159) mtDNA D-loop region showed a positive (+) detection only for day 1 (2 [33.37%] of samples) and day 7 (6 [100%] of samples). Conclusion: In conclusion, the length of exposure to room temperature has an effect on the quality of earphone swab-derived DNA. Decreased levels of earphone swab-derived DNA showed a significance value (p<0.005) with regard to the effect of room temperature exposure
| Item Type: | Article | ||||||
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| Uncontrolled Keywords: | DNA quality, Ear phone swabs, Forensic Evidence, Mitochondrial DNA, Room temperature | ||||||
| Subjects: | R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA1001-1171 Forensic medicine. Medical jurisprudence. Legal medicine |
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| Divisions: | 01. Fakultas Kedokteran > Ilmu Forensik | ||||||
| Creators: |
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| Depositing User: | arys fk | ||||||
| Date Deposited: | 29 Sep 2020 05:07 | ||||||
| Last Modified: | 27 Apr 2021 01:10 | ||||||
| URI: | http://repository.unair.ac.id/id/eprint/99469 | ||||||
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