Agung Sosiawan, - and Ahmad Yudianto, - and Abdul Hadiq Furqoni, - and Simon Martin Manyanza Nzilibili, - and Indah Nuraini, - (2019) Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene. Egyptian Journal of Forensic Sciences, 9 (38). pp. 1-10. ISSN 20905939

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Abstract

Background Sibling relation over chosen STR loci and localized database is yet to be developed in Indonesia like into many other countries despite the demand of varied ethnic population, catastrophes and civil conflict that call forensic preparedness. The targeted application includes human genomic typing and profile, kinship/sibship relation, forensic applications, individual identification, and or claneology tracing. Consequently, this study devotes on sibship in assessing and establishing preferable STR loci and allelic sharing extent amongst Madurese full sibling. Materials and methods One hundred blood samples were used to examine sibling relationship among 50 full siblings (25 pairs) assigned from a total of 100 individuals of 25 Madura families. Sibship relation was done by STR technique profile by 12 loci (CSF1PO, F13B, FES, TH01, TPOX, vWA, D5S818, D7S820, D8S1179, D13S317, D16S539, and D18S51) and a sex-typing amelogenin gene locus. Results From 300 observations (25 pairs of full-sibling × 12 loci) of genotyped STR allele, of allele sharing, has found 4 STR loci: FES, CSF1PO, F13, and D8S1179 with high-shared allelic frequency in two alleles sharing among twelve STR loci by 52%, 52%, 44%, and 40% respectively. As for the four main loci of full sibling with one allele sharing, i.e, D7S820, D18S51, vWA, and THO1, have a sequence of percentages from large to small as follows: 76%, 76%, 72%, and 60%, respectively. Furthermore, STR locus VWA expressed null shared allele in 24 allele-sharing category. Likewise, F13 and FES both typed null allele in zero allele-sharing category among these full sibling. Conclusion Through the use of 12 autosomal STR loci, the study has signified the strength of 2 shared allele evidence by 51.2% in establishing sibship and recommend D7S820, VWA, TH01, and D18S51 as STR loci of choice when typing sibling among Madurese.

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