Tin Myo Thant (2020) Secondary Metabolites From Myanmar Medicinal Plant (Clausena excavata) And Their Antioxidant, Antidiabetic, And Anticancer Activitites. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

In the course of searching bioactive constituents from Myanmar medicinal plant (Clausena excavata), a total of 17 isolated and modified compounds were reported from this species. Among them one new compound, TMT-4, (excavatin-A) was isolated together with six known compounds; TMT-1 (nordentatin), TMT- 2 (dentatin), TMT-3 (heptaphylline), TMT-5 (mukonine), TMT-6 (xanthoxyletin), and TMT-7 (7-hydroxyheptaphylline) were naturally isolated from C. excavata. Moreover, 10 new pyranocoumarin benzoate derivatives (TMT-1a to TMT-1j), were semi-synthesized from TMT-1 (nordentatin). All structures were elucidated by using spectroscopy methods. All the isolated and modified compounds were tested on their antioxidant activity by DPPH assay, antidiabetic activity by using α-glucosidase inhibition assay and cytotoxicity by MTT assay. Among isolated compounds, TMT-1 showed the highest antioxidant activity with IC50 values 0.02 mM. Moreover, TMT-1 exhibited strong inhibition activity against maltase (IC50 5.45 µM) and sucrase (IC50 43.57 µM), whereas TMT-2 showed high inhibition against maltase and yeast enzymes. Moreover, inhibition activity modified compounds against on yeast α-glucosidase were performed and compared with the parent compound (TMT-1). Of modified compounds, TMT-1b exhibited highest inhibition against yeast α-glucosidase with IC50 values 1.54 mM (where, acarbose IC50 values 7.57 mM was used as standard) more inhibit than parent compound TMT-1 (nordentatin, 37.62 mM) on yeast α-glucosidase enzymes. In addition, the evaluation of cell viability of Hela (cervical cancer) and T47D (breast cancer) of isolates and semi-synthesized compounds were performed. Among tested compounds, new compound TMT-4 (excavatin-A) showed the highest cell cytotoxic activity on hela (IC50, 44.5 µM) and on the other hand, TMT-1 (nordentatin) showed the highest values on T47D (IC50 41.3 µM).

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