Ahmad Yudianto and Simon Martin Manyanza Nzilibili (2018) Earphone Swab-Derived 126bp and 143bp mtDNA at D-loop Region - Electrophoretic Band Reaction on Room Temperature. In: Proceedings of the 2nd International Conference Postgraduate School (ICPS 2018). Science and Technology Publications, Lda., Surabaya, pp. 499-503. ISBN 978-989-758-348-3

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Abstract

Exploration of crime scene devices, leftover, and its surrounding is of significance in forensic identification due to its potential harbor of biological evidences. Such harboring of evidences proposes traceability under Locard’s principle. By body use-contact, devices such as earphones potentiate revelation of the unknown by analysis of cerumen or traces logged from ear. Despite of this usability, environmental challenge is suggested to impact the forensic vitality of the logged evidences. To understand the phenomena, this study applied 30 used earphones at 0, 1, 7, 14 and 20 days room temperature exposure. From the study; amplified electrophoretic reaction showed that, 143bp (HVS I, nt 16268-16410) mtDNA D-loop region reacted with a positive (+) detection only for day 1 (4 [66.67%] samples) and day 7 (3 [50%] samples) of room temperature exposure. Meanwhile, visualization of the results of 126bp (HVS II, nt 34-159) mtDNA D-loop region showed a positive (+) detection only for day 1.

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