Dwi Ratna Aristantya, - and Gandul Atik Yuliani, - and Endang Suprihati, - (2016) Uji Reaktivitas Protein 30 kDA Bakteri Aeromonas hydrophila yang Diisolasi dari Ikan Air Tawar dengan Teknik Indirect ELISA. Journal of Basic Medical Veterinary, 5 (1). pp. 25-31. ISSN 2302-6820

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Abstract

This study aimed to determine the reactivity of 30 kDa protein Aeromonas hydraphila. The reactivity is indicated by a binding· between the antigen and antibody of rabbit (Oryctolangus cuniculus) that is based in the optical density score in indirect ELISA technique. The isolated Aeromonas hydrophila was characterized in preyjous research using Western-B lot and SOS-PAGE. method, produc Aerornonas hydraphila protein that stated in 30 kDa has potential immunogenic. Three male rabbits immunized with 1 ml of adjuvant as control, 108 cfu/ ml whole protein of Aeromonas hydraphi la as Pl and 2.Sx107 IU/ml of protein 30 kDA Aeromonas hydrophila as P2 respectively. Immunization was done four times with an interval of 14 days. Antibody titer tested by indirect ELISA technique from collected serum. The result showed that the antibody titer af ter immunization IV (1.550) ·higher than after immunization I (1.341) and pre-immune (1.219). Keywords :Aeromonas hydrophila, protein, immunization, ELISA

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