Antiviral Activity of the dichloromethane extracts from Artocarpus heterophyllus leaver against hepatitis C virus

Fuad Hafid, Achmad and Aoki-Utsubo, Chie and Ayu P, Adita and Adianti, Myrna and Tumewu, Lydia and Widyawaruyanti, Aty and Puji Astuti W, Sri and Sri Wahyuni, Tutik and Inge L, Maria and Soetjipto, Soetjipto and Hotta, Hak (2018) Antiviral Activity of the dichloromethane extracts from Artocarpus heterophyllus leaver against hepatitis C virus. Asian Pacific Journal of Tropical Biomedicine, 7 (7). pp. 633-639. ISSN 2221-1691

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Official URL: https://reader.elsevier.com/reader/sd/pii/S2221169...

Abstract

Objective To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6) μg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) μg/mL and (9.7 ± 1.1) μg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.

Item Type: Article
Uncontrolled Keywords: Hepatitis C virusArtocarpus sp.Artocarpus heterophyllusAntiviral
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsEmail
Fuad Hafid, AchmadUNSPECIFIED
Aoki-Utsubo, ChieUNSPECIFIED
Ayu P, AditaUNSPECIFIED
Adianti, MyrnaUNSPECIFIED
Tumewu, LydiaUNSPECIFIED
Widyawaruyanti, AtyUNSPECIFIED
Puji Astuti W, SriUNSPECIFIED
Sri Wahyuni, TutikUNSPECIFIED
Inge L, MariaUNSPECIFIED
Soetjipto, SoetjiptoUNSPECIFIED
Hotta, HakUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 06 Jul 2019 06:13
Last Modified: 06 Jul 2019 06:13
URI: http://repository.unair.ac.id/id/eprint/84665
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