Sepvian Dewi Kurniawati (2020) Pengaruh Urea Dalam Media Maturasi In Vitro Terhadap Tingkat Maturasi Oosit Sapi. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
This study was aimed to determine the percentage of in vitro oocyte maturation in TCM – 199 media with urea. Cumulus oocyte complexes (COCs) that had been selected from the dissection media were added into the maturation media that contained TCM -199, Hepes, NaHCO3 and Kanamycine . Maturation media were added 0.15 IU/ml PMSG, 0.15 IU/ml hCG and FBS. Each petridish contained three drops of maturation media (300 μl/drops) which were coated with mineral oil and then incubated in a 5% CO2 incubator, with maximum humidity and temperature of 39˚C. Control (P0) was oocyte maturation without the addition of urea, while treatment 1 (P1) and treatment 2 (P2) were oocyte maturation with the addition of urea, 20 mg/dl and 40 mg/dl respectively. Aceto orcein staining showed that oocyte reached metaphase II phase were 51.25, 52.43 and 46.88 % respectively. ANOVA showed that there was no significant difference (p> 0.05) among treatment groups.
Item Type: | Thesis (Skripsi) | |||||||||
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Additional Information: | KKC KK KH.05/20 Kur p | |||||||||
Uncontrolled Keywords: | In vitro maturation, Urea, Bovine Oocyte | |||||||||
Subjects: | T Technology > TS Manufactures > TS2284-2288 Animal feeds and feed mills. Pet food industry | |||||||||
Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
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Depositing User: | Dwi Marina | |||||||||
Date Deposited: | 25 Feb 2020 03:49 | |||||||||
Last Modified: | 25 Feb 2020 03:49 | |||||||||
URI: | http://repository.unair.ac.id/id/eprint/94462 | |||||||||
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