Sepvian Dewi Kurniawati (2020) Pengaruh Urea Dalam Media Maturasi In Vitro Terhadap Tingkat Maturasi Oosit Sapi. Skripsi thesis, UNIVERSITAS AIRLANGGA.
![[img]](https://repository.unair.ac.id/style/images/fileicons/text.png) |
Text
1. HALAMAN JUDUL.pdf Download (934kB) |
|
Text
2. ABSTRACT.pdf Download (305kB) |
|
Text
3. DAFTAR ISI.pdf Download (337kB) |
|
Text
4. BAB 1 PENDAHULUAN.pdf Download (195kB) |
|
Text
5. BAB 2 TINJAUAN PUSTAKA.pdf Restricted to Registered users only until 25 February 2023. Download (512kB) | Request a copy |
|
Text
6. BAB 3 MATERI DAN METODE.pdf Restricted to Registered users only until 25 February 2023. Download (447kB) | Request a copy |
|
Text
7. BAB 4 HASIL PENELITIAN.pdf Restricted to Registered users only until 25 February 2023. Download (214kB) | Request a copy |
|
Text
8. BAB 5 PEMBAHASAN.pdf Restricted to Registered users only until 25 February 2023. Download (342kB) | Request a copy |
|
Text
9. BAB 6 KESIMPULAN DAN SARAN.pdf Restricted to Registered users only until 25 February 2023. Download (172kB) | Request a copy |
|
Text
10. DAFTAR PUSTAKA.pdf Download (297kB) |
|
Text
11. LAMPIRAN.pdf Restricted to Registered users only until 25 February 2023. Download (528kB) | Request a copy |
Abstract
This study was aimed to determine the percentage of in vitro oocyte maturation in TCM – 199 media with urea. Cumulus oocyte complexes (COCs) that had been selected from the dissection media were added into the maturation media that contained TCM -199, Hepes, NaHCO3 and Kanamycine . Maturation media were added 0.15 IU/ml PMSG, 0.15 IU/ml hCG and FBS. Each petridish contained three drops of maturation media (300 μl/drops) which were coated with mineral oil and then incubated in a 5% CO2 incubator, with maximum humidity and temperature of 39˚C. Control (P0) was oocyte maturation without the addition of urea, while treatment 1 (P1) and treatment 2 (P2) were oocyte maturation with the addition of urea, 20 mg/dl and 40 mg/dl respectively. Aceto orcein staining showed that oocyte reached metaphase II phase were 51.25, 52.43 and 46.88 % respectively. ANOVA showed that there was no significant difference (p> 0.05) among treatment groups.
| Item Type: | Thesis (Skripsi) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Additional Information: | KKC KK KH.05/20 Kur p | |||||||||
| Uncontrolled Keywords: | In vitro maturation, Urea, Bovine Oocyte | |||||||||
| Subjects: | T Technology > TS Manufactures > TS2284-2288 Animal feeds and feed mills. Pet food industry | |||||||||
| Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
| Creators: |
|
|||||||||
| Contributors: |
|
|||||||||
| Depositing User: | Dwi Marina | |||||||||
| Date Deposited: | 25 Feb 2020 03:49 | |||||||||
| Last Modified: | 25 Feb 2020 03:49 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/94462 | |||||||||
| Sosial Share: | ||||||||||
Actions (login required)
 |
View Item |