Wijayanti, Tri Arini, NIM. 050312704 (2007) OPTIMASI FASE GERAK METANOL � AIR UNTUK ANALISIS KUANTITATIF CAMPURAN PARASETAMOL DAN KODEIN FOSFAT DENGAN METODE KCKT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Determination of Paracetamol and Codeine Phosphate simultaneously using the reversed phase High-Performance-Liquid-Chromatography (HPLC) have been performed. The mobile phase solution obtained can separate parasetamol from codeine phosphate with good resolution (Rs = 2,704), this mobile phase were a mixture of methanol and phosphate buffer 20 mM pH 2,5 (15:85). The condition of HPLC used were zorbax C8 column, temperature 27°C, flow rate 1,5 ml/minutes and detected by DAD (λ = 284 nm). Linierity of parasetamol and codeine phosphate were good (r paracetamol 0,9999 with Vxo = 0,51 % and r codeine phosphate 0,9999 with Vxo = 1,22 %). Precision instrument for paracetamol and codein phosphate were 0,77 % and 0,79 % respectively. Accuracy of the methods were in the range of accepted limits ( 98 – 102%), average recovery of paracetamol and codeine phosphate in the simulation matrix were 99,99 % and 100,5 % respectively. Precision methods of Parasetamol 0,64% and Codeine phosphat 0,75%. The concentration of paracetamol and codeine phosphate in sample tablet were 100,8% and 99,52% respectively, that are fulfill of USP XXIV requirement ( 90 – 110 % )

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