Abdulloh Machin (2020) Penghambatan Proses Kematian Sel Neuron Menggunakan Camillia Sinensis Dengan Bahan Aktif Egcg Pada Rattus Norvegicus Model Stroke Iskemik Akut. Disertasi thesis, UNIVERSITAS AIRLANGGA.

	Text (HALAMAN JUDUL) 1. HALAMAN JUDUL.pdf Download (189kB)
	Text (ABSTRACT) 2. ABSTRACT.pdf Download (62kB)
	Text (DAFTAR ISI) 3. DAFTAR ISI.pdf Download (80kB)
	Text (BAB I PENDAHULUAN) 4. BAB 1 PENDAHULUAN.pdf Download (64kB)
	Text (BAB 2 TINJAUAN PUSTAKA) 5. BAB 2 TINJAUAN PUSTAKA.pdf Restricted to Registered users only until 12 January 2024. Download (928kB) | Request a copy
	Text (BAB 3 KERANGKA KONSEPTUAL DAN HIPOTESIS PENELITIAN) 6. BAB 3 KERANGKA KONSEPTUAL DAN HIPOTESIS PENELITIAN.pdf Restricted to Registered users only until 12 January 2024. Download (142kB) | Request a copy
	Text (BAB 4 METODE PENELITIAN) 7. BAB 4 METODE PENELITIAN.pdf Restricted to Registered users only until 12 January 2024. Download (154kB) | Request a copy
	Text (BAB 5 HASIL PENELITIAN) 8. BAB 5 HASIL PENELITIAN.pdf Restricted to Registered users only until 12 January 2024. Download (400kB) | Request a copy
	Text (BAB 6 PEMBAHASAN) 9. BAB 6 PEMBAHASAN.pdf Restricted to Registered users only until 12 January 2024. Download (252kB) | Request a copy
	Text (BAB 7 KESIMPULAN DAN SARAN) 10. BAB 7 KESIMPULAN DAN SARAN.pdf Restricted to Registered users only until 12 January 2024. Download (23kB) | Request a copy
	Text (DAFTAR PUSTAKA) 11. DAFTAR PUSTAKA.pdf Download (166kB)
	Text (LAMPIRAN) 12. LAMPIRAN.pdf Restricted to Registered users only until 12 January 2024. Download (418kB) | Request a copy
	Text (PERMOHONAN EMBARGO) PERMOHONAN EMBARGO.pdf Restricted to Registered users only Download (939kB) | Request a copy

Abstract

Background: Stroke is the most prevalent neurological disorders in the world. During ischemic stroke there is increasing oxidative stres that will cause cell death through apoptosis and necroptosis pathways. Methods: We perform in vivo study using male Rattus Novergicus within 5 groups, control MCAO, EGCG 10 mg/kgBB, EGCG 20 mg/kgBB, EGCG 30 mg/kgBB, and extract green tea 30 mg/kgBB. Before performing MCAO models all study subject examine for Ladder Rung, and Y-Maze. We perform MCAO model using clamping carotid artery for 180 minutes. All groups are treated for 7 days and at day 7th we perform ladder rung and Y maze examination before research subject is sacrifice and examine HMGB1 using ELISA methods, and IHC for HO-1, TNFR1, RIP3, BCL-2 and Caspase-3. Result: There is significant different in all intervention group compared to control group on HO-1 (p<0,05). There is no significant different in all groups compared to control group in HMGB1. There is also significant different in all intervention group started at EGCG 20 mg/kgBW compared to control group on TNFR1 (p<0,05), significant different for RIP3 started at EGCG 20 mg/kgBW and extract green tea group (p<0,05), BCL-2 for all intervention group (p<0,05), Caspase-3 at EGCG 30mg/kgBW (p=0,004) and green tea extract group (p=0,019). There are no significant different on ladder rung at days 7th for all groups. There is also significant different in Y-Maze Score at green tea extract groups (p=0,048). There is significant correlation between HO-1 and BCL-2 (r=-0,655; p=0,000), BCL-2 and Caspase-3 (r=-0,5 ; p=0,000), Caspase-3 and Y-Maze (r=0,332; p=0,001), TNFR1 and RIP3 (r = 0,551; p=0,000) and we didn’t find correlation between HMGB1 and TNFR1 (r=0,029; p=0,838), RIP3 and Y-Maze (r=0,18; p=0,167). Conclusion: Green tea with its active coumpound EGCG can inhibit neuronal cell death through apoptosis and necroptosis pathways in MCAO models.

Actions (login required)

View Item