Ernawati (2020) Mekanisme Kerja Xanthone Sebagai Protektor Pada Spermatozoa Dan Testis Mencit (Balb/C) Yang Diinduksi Dengan 2-Methoxyethanol. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Oxidative stress has a very important role in the mechanism of action of 2 -Methoxyethanol (2-ME) in spermatozoa cells and testicular damage. The xanthones have a very strong antioxidant effect. Objective: This research aims to investigate the mechanism of action of xanthone in against 2-methoxyethanol (2-ME) induced spermatozoa and testicular toxicity in mice. Methods: Thirty-five male mice divided into 5 groups: negative control (mice were given daily with CMC 0.5 % and aquadest for 38 days); positive control (mice were given daily with CMC 0.5 % for 38 days, and on the 4th day, were given 2-ME 200 mg/kg BW orally once in a day for 35 days); and the treatment group ( mice were given the xanthone 60 mg, 120 mg, and 240 mg/kg BW orally once in a day for 38 days, and on the 4th day, were given 2-ME 200 mg/kg BW one hour after the xanthone administration). After 38 days, the testis tissues were collected to evaluate the immunohistochemical of the expression of malondialdehyde (MDA), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) in the spermatogenic cell, Leydig cell, and Sertoli cell. Testis tissue was also taken to histological evaluations of the spermatogenic cell, Leydig cell, Sertoli cell number, the thickness of epithelium and diameter of tubules seminiferous. The concentration, morphology, motility, and viability of spermatozoa in the epididymis were also evaluated. Results: The 2-ME administration significantly decreased the expression of SOD, GPx, and increased the expression of MDA in the spermatogenic cell, Leydig cell and Sertoli cell. The 2-ME also significantly decreased spermatogenic cell, Leydig cell, Sertoli cell number, the thickness of epithelial and diameter of tubules seminiferous. The concentration, morphology, motility, viability of spermatozoa cell were also decreased by 2-ME. However, on immunohistochemical examination, xanthone administration can significantly inhibit decreased SOD, GPx expression, and inhibit increased MDA expression in spermatogenic cells, Leydig cells and Sertoli cells in the testes of mice induced with 2-ME. The histopathological evaluation, xanthone administration significantly inhibited the decreased spermatogenic cell, Leydig cell, Sertoli cell number, the thickness of epithelial and diameter of tubules seminiferous on testis induced by 2-ME. Xanthone also inhibits decreased concentration, morphology, motility, the viability of spermatozoa of epididymis cell which induced by 2-ME. Conclusion: The mechanism of action of xanthones in protecting on 2-ME-induced mice spermatozoa and testicular damage is through its inhibition of increased MDA expression, and its inhibition of decreased SOD and GPx expression

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