Nurwasis and Gatut Suhendro and I Ketut Sudiana (2019) The Role of SOD, Catalase, HSP-27, HSP-70, and TNF-? Expression in Apoptosis of Retinal Ganglion Cells After Intra Ocular Pressure Increase on Rattus Norvegicus. Indian Journal of Public Health Research & Development, 10 (7). pp. 1174-1178. ISSN 09760245

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Abstract

The aim of this study is to investigate the involvement of Super Oxide Dismutase (SOD), Catalase, Heat Shock Protein 70 (HSP 70), Heat Shock Protein 27 (HSP 27) Retinal Ganglion Cells (RGCs) and Tumor Necrosis Factor ? (TNF-?) microglia in apoptosis RGCs and RGCs survival after transient periode of pressure induced ischemia/reperfussion injury of rat retina.The study design was randomized post test only control groups. Thirty one Sprague Dawley (SPD) rats were divided into 4 groups. 1 control group and 3 experimental groups. Experimental groups were induced by transient elevation of intraocular pressure (IOP) 110-130 mmHg for 45 minutes (7 rats), 60 minutes (7 rats), 75 minutes (7 rats). 28 rats were terminated at day 7 and 3 rats were terminated at first day for histology and immunohistochemistry staining examination. Result from the Expression of SOD, HSP 70 GSRs and TNF-? microglia were significantly different. p=0,046 and B=0.380 for SOD; p=0.030 and B=0,411 for Hsp 70; p=0.007 and B=0,501 for TNF-?. Expression of catalase and HSP 27 GRCs were not significantly different. p=0.203 for catalase; p=0.083 for Hsp 27. The increased expression of HSP 70 indicated strong correlation with increased level of apoptosis RGCs, p=0.046 and B=0,473. In conclusion, the result demonstrated that RGCs apoptosis survival in glaucoma correlates strongly with transient elevated IOP and is significantly associated with IOP induced changes in expression HSP 70 RGCs.

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