Salsabilla Abani and Widjiati and Erma Safitri and Maslichah Mafruchati and Djoko Legowo and Lilik Maslachah (2020) Effect of Green Tea (Camellia sinensis) Ethanol Extract Administration on The Number of Spermatogenic Cells of Male Mice (Mus musculus) Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin. International Journal of Pharmaceutical Research, 12 (3). pp. 1170-1174. ISSN 0975-2366

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Abstract

One of the cause of infertility in male reproduction is contamination of hazard chemical. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is known as most toxic chemical which can impair the reproductive system and cause low count of spermatogenic cells. The purpose of this study was to know the effect of green tea (Camellia sinensis) ethanol extract on the number of spermatogenic cells of male mice (Mus musculus) exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. This research used 25 male mice which divided into 5 groups of treatment i.eC(-) received aquadest only, C(+). T(1), T(2), and T(3) injected with TCDD 0,14 µg per mice and continued with the treatment. C(+) treated with Epigallocatechin gallate (EGCG) 1,2 mg/kg BW, T(1), T(2) and T(3) given green tea ethanol extract with a dose of 1 mg/kg BW ; 2 mg/kg BW and 4 mg/kg BW respectively orally 0.1 ml for 53 days. After that the mice sacrificied and the testis were taken for making histopathology slides using HE staining. The results showed that there are significant difference (P <0.05) of spermatogenic cells among the treatment group where T(1) with a mean of 100.68 ± 1.91, T(2) with a mean of 136.32 ± 2.33 and T(3) group with an average of 166.84 ± 3.40. Between T(3) and C(-) with a mean of 169.72 ± 2.67 showed there are no significant difference. The conclusion is green tea ethanol extract could maintain the number of spermatogenic cells Keyword: Green tea, Spermatogenic cells, TCDD

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