Salsa Ajeng Kirana (2020) Isolation And Identification Of Salmonella sp On Duck’s Farm In Jombang East Java. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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6. CHAPTER 3 RESEARCH METHOD.pdf Restricted to Registered users only until 8 March 2024. Download (184kB) | Request a copy |
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Abstract
This study was aimed to identify Salmonella sp. on duck’s farm. Samples are taken from duck’s farm in Jombang. 140 samples of duck’s cloacal swab are taken from broiler duck farm in Ds. Tembalang, Jombang, East Java, the rest of 63 samples taken from layer duck’s farm in Ds. Tunggurono, Jombang, East Java. Cloacal swab’s sample then stored in 15 ml Buffered Peptone Water (BPW) and incubated in 37oC for 24 hours. Then, the soaked sample stored in tube consist of Selenite Cystine Broth (SCB) and incubated in 37oC for 24 hours. After being incubated, the soaked samples inoculated with ose on Xylose Lysine Deoxycholate (XLD) agar and incubated in 37oC for 24 hours. After incubated, all samples taken for biochemical test for further identification with Triple Sugar Iron Agar (TSIA) media. This treatment and test apply to 203 sample from 2 different duck’s farm. The result of conventional test to identify Salmonella sp. show that 179 (88.17%) samples on XLD agar and 152 (74.87%) samples on TSIA positive for Salmonella sp. The result of PCR assay show that 107 (52.70%) samples identified as Salmonella sp.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Additional Information: | KKC KK KH 25-21 Kir i | |||||||||
| Uncontrolled Keywords: | Salmonella sp, Salmonellosis, Duck, Polymerase Chain Reaction (PCR) | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.9 Veterinary epidemiology. Epizootiology |
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| Divisions: | 06. Fakultas Kedokteran Hewan > Ilmu Kedokteran Hewan Dasar | |||||||||
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| Depositing User: | prasetyo adi nugroho | |||||||||
| Date Deposited: | 08 Mar 2021 06:42 | |||||||||
| Last Modified: | 08 Mar 2021 06:42 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/104687 | |||||||||
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