Lutfa Qurrota A yun (2020) Penentuan Fraksi Aktif Antimalaria Dari Ekstrak Diklorometana Kulit Batang Artocarpus Sericicarpus Dengan Target Penghambatan Pada Enzim Malate Quinone Oxidoreductase Plasmodium Falciparum (Pfmqo). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Malaria is an infectious disease that still endemic in Indonesia. Exploration of active ingredients from nature is one of the approaches in finding new antimalarials. Artocarpus sericicarpus stembark dichloromethane extract has previously been reported as a potential antimalarial drug. The aim of this study is to obtain an active antimalaria fraction of A. sericicarpus stembark dichloromethane extract with the target inhibition on Malate Quinone Oxidoreductase Plasmodium falciparum (PfMQO). Stembark of A. sericicarpus was extracted using an ultrasonic-assisted extraction method with n-hexane, dichloromethane, and methanol solvents. Fractionation of A. sericicarpus stembark dichloromethane extract was conducted by open column chromatography method. The antimalarial assay was determined using LDH assay against P. Falciparum and MQO assay to determine target inhibition of the MQO enzyme. There are 12 fractions of A. sericicarpus stembark dichloromethane extract, fraction-3,4,5,6,7,8 and 12 has antimalarial activity IC50 <5 μg/mL with strong active category. Fraction-6 has the highest potential antimalarial drug, inhibition concentration (IC50) against P. falciparum at 1.53 ± 0.04 μg/mL and has the ability to inhibit MQO enzyme at 6.42 ± 0.03 μg/mL. Fraction-6 contains flavonoid, polyphenol, and terpenoids compound which provides the antimalarial activity. An MQO assay for fraction-3,4,5,7,8,12 is required, subfractination and isolation of fraction-6 is recommended to obtain new antimalarial compounds by inhibiting MQO enzyme in mitochondria.

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