The Effect of Hemolysis on Hematology Laboratory Test Repository

The Effect of Hemolysis on Hematology Laboratory Test

Yetti Hernaningsih and Dewintha Airene Novianti (2020) The Effect of Hemolysis on Hematology Laboratory Test. In: Advances in Health and Disease. Nova Science, New York, pp. 117-148. ISBN 978-1-53618-484-6

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Abstract

Hemolysis in blood samples is a problem that is commonly found in laboratories. This has a potential effect on the qualiy of blood tests, completion time or TAT (turn around time), and creates discomfort for patients due to repeated sampling. Hemolysis can occur in vitro or in vivo. In vitro hemolysis can occur due to lysis of red blood cells during the sample collection and handling of blood samples. In vitro hemolysis is a result of pre-analytical causes associated with sample collection, jarring transportation methods, extreme temperature, sample handling, delayed processing, and prolonged storage. In vivo hemolysis occurs if the rate of erythrocyte damage increases, thereby reducing the life span of erythrocytes. Hemolysis results in a decrease in the number of RBCs and HCT values due to lysis. Hematological instruments usually lyse the sample before measuring the HGB, the number of PLT, the number of WBC, and the number of WBC differential cells, so these values are usually not affected by hemolysis. In samples with hemolysis, the ESR values will decrease. In the ESR test with the Westergreen method, it is often found that hemolysis samples are difficult to assess because of unclear boundaries. The mechanism for shortening APTT in hemolysis samples has not been confirmed. This is thought to be caused by the release of phospholipids from erythrocytes and intracellular substances from leukocytes and platelets which can activate Cascade coagulation. Other literature states that activating the freezing cascade will cause PPT shortening and decreased fibrinogen levels, whereas APTT can extend or shorten the levels depending on whether there is activation or loss of fibrinogen. Hemolysis samples that are immediately examined may experience coagulation activation, so the APTT results are shortened.

Item Type:

Book Section

Uncontrolled Keywords:

Hemolysis, Hematology Laboratory Test

Subjects:

R Medicine > R Medicine (General)
R Medicine > RB Pathology > RB37-56.5 Clinical pathology. Laboratory technique

Divisions:

01. Fakultas Kedokteran > Patologi Klinik

Creators: