Alpha Fardah Athiyah, - and Nur Aisiyah Widjaja, - and Pramira Fitri, - and Ariani Setiowati, - and Andy Darma, - and Reza Ranuh, - and Subijanto Marto Sudarmo, - (2019) Effects of a multispecies synbiotic on intestinal mucosa immune responses. Iranian Journal of Microbiology, 11 (4). pp. 300-304. ISSN 2008-3289

	Text (Peer Review) Effects of Multispecies.pdf Download (1MB)
	Text (Similarity) 3. Effects of a multispecies synbiotic on intestinal mucosa immune responses.pdf Download (1MB)
	Text (Bukti Korespondensi) Bukti Korespondensi 3.pdf Download (1MB)
	Text (Peer Review) C12 Peer Review.pdf Download (2MB)
	Text (Artikel) C12 Artikel.pdf Download (4MB)
	Text (karil) 12.pdf Download (19kB)

Abstract

Background and Objectives: Probiotics and prebiotics are known to regulate immune responses. A synbiotic is a product that combines probiotics and prebiotics in a single dosage form. In this study, we attempt to present the effects of a multispecies synbiotic on intestinal mucosa immune responses after exposure to Escherichia coli O55:B5 lipopolysaccharide (LPS). Materials and Methods: Totally 21 male Balb/c mice were randomly classified into two groups. The K-I group received LPS and a synbiotic, and the K-II group received LPS alone. The synbiotic was administered for 21 consecutive days, whereas LPS was administered once on the 15th day. Specifically, a synbiotic containing 1 × 109 colony forming units (CFUs) of the probiotic combination of Lactobacillus acidophilus PXN 35, L. casei subsp. casei PXN 37, L. rhamnosus PXN 54, L. bulgaricus PXN 39, Bifidobacterium breve PXN 25, B. infantis PXN 27 and Streptococcus thermophilus PXN 66 and the prebiotic fructo-oligosaccharide was administered through an orogastric tube. Immunohistochemistry was performed to measure immunoglobulin A (IgA) levels for humoral immune responses and CD4+ and CD8+ levels for cellular immune responses. Results: An independent-samples t-test revealed significant increases of the numbers of IgA- (p = 0.027) and CD4-expressing cells (p = 0.009) but not the number of CD8-expressing cells in the K-I group compared with those in the K-II group. Conclusion: The multispecies synbiotic had immunoregulatory effects on IgA and CD4 expression in LPS-exposed mice.

Item Type:	Article
Uncontrolled Keywords:	Synbiotic, Lactobacillus, Bifidobacterium, Streptococcus, Immune response
Subjects:	R Medicine > R Medicine (General) R Medicine > RJ Pediatrics
Divisions:	01. Fakultas Kedokteran > Ilmu Kesehatan Anak (Sub Spesialis)
Creators:	Creators NIM Alpha Fardah Athiyah, - NIDN0023087301 Nur Aisiyah Widjaja, - NIDN8935700020 Pramira Fitri, - UNSPECIFIED Ariani Setiowati, - UNSPECIFIED Andy Darma, - UNSPECIFIED Reza Ranuh, - NIDN8811010016 Subijanto Marto Sudarmo, - NIDN0010064803
Depositing User:	arys fk
Date Deposited:	27 May 2021 02:55
Last Modified:	29 Nov 2022 00:47
URI:	http://repository.unair.ac.id/id/eprint/107339
Sosial Share:

Actions (login required)

View Item